A compar aison between intravenous and intraperitoneal injection of human stem cell (cd34+) with/ or without mesenchymal stem cell in albino rats

This study aimed to assess the in vivo engraftment and proliferation of purified human haemopoietic stem cell infusion or combined infusion containing haemopoietic stem cells plus mesenchymal stem cells in albino rats through intravenous and intraperitoneal infusion. Twenty female albino rats were used for human cells engraftment, whereas 10 served as a control group. 20 to 40 ml of cord blood samples and length of 12 cm of umbilical cords for Wharton jelly samples were obtained from 10 newborns delivered after full term following ethical consent. Ficoll Paque was used to obtain the mononuclear cells from cord blood samples while trypsin enzymatic treatment was used for Wharton jelly samples. Purification of CD34+ cells was done using RosetteSep and EasySep techniques. The expression and count of CD44+, CD34+and CD45+ cells was analyzed using flowcytometer. The study confirmed the success of in vivo engraftment, self renewal and proliferation of immune isolated human umbilical cord HSC/CD34+ by albino rats and differentiation to CD45+ cells, beside the highly increase of the harvested mononuclear cells from the spleen and bone marrow without any case of GvHD within 3 weeks period. Moreover, best engraftment obtained from the infusion that contains CD34+ cells and MSCs.