Comparative Analysis Of Antibacterial, Antioxidant And Photosynthetic Activity Of Azadirachta Indica, Rosa Indica And Moringa Oliefera Cultivars

In the present study, three plants viz. Rosa indica (petals), Azadirachta indica (leaves) and Moringa oleifera (leaves) were screened for potential antibacterial and antioxidant activity. To evaluate these activities two organic solvents i.e. acetone and ethanol were used to extract the antibacterial and antioxidant compounds from plant sample. Antibacterial activity was tested against two gram positive i.e. Staphylococcus aureus, Bacillus pumilis and two gram negative i.e. Klebsiella pneumoniae, Escherichia coli bacterial strains by agar well diffusion method. The ethanolic extracts of red rose petals were found to be most effective against all the pathogens tested. Antioxidant activity of all three plants was screened by measuring total phenolic content, total flavonoids and by free radical scavenging assay using DPPH. Significant differences in DPPH scavenging activity were reported between the species investigated, ranging from 74.72% to 83.40%. The total phenol content of the investigated species ranged from 74 to 96 mg CE/ g extract while flavonoid content ranged between 39 to 52 mg QE/g extract. In addition photosynthetic pigments (Chl A, Chl B, and carotene) were also determined for all three plants under study.