Cultural studies, production of nitrogen, total sugar, pigments and lipid by chlorogloeopsis fritschii bta9016 a cyanobacterium from paddy fields of Allahabad, India

Cyanobacterium Chlorogloeopsis fritschii Mitra 1950 displayed irregular rounded and elongated cell packets during in-situ germination and controlled culture conditions. The strain was investigated for acetylene reduction activity and also assessed for lipid profiling and fatty acid composition by Nile red method followed GC-FID using Supelco standard. Investigation expressed high and considerable amount of acetylene reduction activity i.e., 4.47 and 11.27 nmole C2H4/µg of Chl-a/hr during 15th and 30th days growth phase respectively. Investigated organism also rigorously worked out for the production and release of pigments, total soluble proteins, total sugar and ammonia excretion during mid and last phase of growth cycle. The 16S rRNA sequences was compared with the retrieved cultures from NCBI GenBank database. Phylogenetic analysis was largely consistent which was obtained from 16S rRNA gene sequence analysis. Phylogenetic trees was constructed by neighbour joining and the findings indicate that the genus Chlorogloeopsis fritschii with accession number KJ562182.1 was distantly related to the genus Nostochopsis and Fischerella.