An experiment was laid out at Krishi Vigyan Kendra, Madurai during 2019-21. The aim of this study is to induce off season flowering (Nov- Feb) in Jasminum sambac Ait. cv. Gundu malli through pruning, chemical manipulation and to find out the suitable chemical for induction of off season flowering in Jasminum sambac Ait. cv. Gundu malli. The following treatments were imposed. The main plot treatments viz., The Pruning was done during the month of August (M1), September (M2) and last week of November (control). The sub plot treatments viz., 0.6 g a.i /m2 of Paclobutrazol (S1), 150 ppm of Mepiquat chloride(S2), 1.0 % of Nitrobenzene(S3), 1.0% of Thio urea (S4) and 1000 ppm and 4% of Cycocel + Humic acid respectively (S4). It was laid out in split plot design with three replications and the results were statically analyzed. Morphological, physiological and yield parameters could be recorded during 2019-21. Among the different pruning and chemical treatments, Among the different pruning and chemical treatments, September pruning (M2) + Thio urea (1.0%) (S4) recorded maximum Plant height (59.50 cm), No. of productive shoots/plant ( 45.50 Nos.), Duration of flowering ( 110.50 Days), Chlorophyll “a” (0.58 mg g-1), Chlorophyll “b”content (0.48 mg g-1), Total phenol content (4.62 µg/g), Corolla tube length (1.21cm), Length of flower bud (2.51cm), Diameter of the flower bud (6.25 mm), Number of flower buds per cyme (8.30 Nos.), Number of cymes per plant (92.50 Nos.), Number of flower buds per plant (230.21 Nos.), Estimated flower yield/plant (55.15 g/plant), flower yield (0.82 kg/plot), estimated flower yield (0.39 t/ha) and shelf life (12.55 days). Whereas, Days taken to initiate flowering i.e. bud initiation (30.50 Days) and Days taken for peak flowering (88.50 Days) were recorded September pruning (M2) +Paclobutrazol (0.6 g a.i /m2)(S1) However, September pruning (M2) + Nitrobenzene (1.0 %) (S3) recorded maximum number of flower bud (8.30 nos.) and weight of hundred flower buds (24.54 g) during 2019-20. During 2020-21 Among the different pruning and chemical treatments, September pruning (M2) + Cycocel + Humic acid (S5) recorded maximum Plant height (46.50 cm), No. of productive shoots/plant ( 25.50 Nos.), Duration of flowering ( 69.50 Days), Chlorophyll “a” (0.59 mg g-1), Chlorophyll “b”content (0.46 mg g-1), Total phenol content (3.62 µg/g), Corolla tube length (1.17 cm), Length of flower bud (2.24 cm), Diameter of the flower bud (6.50 mm), Number of flower buds per cyme (8.50 Nos.), Number of cymes per plant (65.50 Nos.), Number of flower buds per plant (195.50 Nos.), flower yield /plant and flower yield per plot were recorded 0.76 kg 5.75 kg respectively.