Microbial bio-burden in health colleges at Najran region, southwest of Saudi Arabia

The experiments aimed to describe the Types and the density of bacterial species and fungi on the surface of small sized clinics, clinical laboratories, classrooms and halls. One hundred and sixteen samples were collected using sterile cotton swabs that are soaked in sterile peptone water broth and the swabs were rubbed into a quarter of 20cm2 on the surfaces. After cultivation of the samples on appropriate growth, media a series of steps for purification and identification of microbial species were conducted. The surfaces within the clinics and the laboratories showed high microbial burden. Staphylococcous aureus and the Sphingomonas paucimobilis were the microorganisms most frequently found on the surfaces. Cleaning and sterilization of the surfaces reduce the microbial content largely.