In vitro propagation of dendrocalamus asper and testing the clonal fidelity using rapd and issr markers

An efficient and reproducible protocol for the large-scale propagation of Dendrocalamus asper was achieved using nodal segments as explants. .High frequency of multiple shoots was induced on Murashige and Skoog’s (MS) medium supplemented with Benzyl amino purine, BAP (8.86 µM) and adenine sulfate (13.5 µM). Regular sub-culturing carried out every 3 weeks on fresh shoot multiplication medium provided long term shoot cultures. Rooting (up to 90 %) was readily achieved upon transferring the shoot clumps (3-4 shoots) onto MS medium supplemented with Indole butyric acid, IBA (14.76 µM) and Naphthalene acetic acid, NAA (3.67 µM). In vitro raised plants were hardened in green house and successfully established in the field conditions, where they exhibited normal growth. Random amplified polymorphic DNA, RAPD and Inter simple sequence repeat (ISSR) markers were used to establish genetic uniformity in micropropagated plants. One hundred thirty three monomorphic fragments derived in 44 (34 RAPD; 14 ISSR) markers based fingerprinting confirmed genetic uniformity in in-vitro raised plants and mother plants.