Approach for lens molecular structure changes in ATP rat’s model of retinal degeneration

Intravitreal administration of adenosine triphosphate (ATP) has been found to be effective for inducing photoreceptor death and loss of retinal function. Due to lack of studies that investigate the changes consumed the lens during early steps of retinal degeneration, the aim of the present search is to give an approach for lens molecular structure changes in ATP rats model of retinal degeneration by using fourior transformer infrared technique. Fifty healthy albino Wistar rats were divided into 4 groups: Group I served as the control group and received intravitrial injection of saline. All other groups were received a single intravitreal injection of 2µL of 1 M ATP and decapitated after 1, 3 and 6 days, respectively. The results indicated that NHOH region was found to be dramatically affected in particular after one day of ATP injection where more vibrational mode was observed relative to control. These reflex more conformational changes which involve moiety of lens fiber. The detection of asymNH after 3 and 6 days of ATP injection indicated the increase of disorder of lens fiber. CH region shows that the symmetric vibration mode (symCH3) is sensitive to ATP injection as indicated by the fluctuation change in its band frequency and this band is associated with lens lipid molecule. The phosphate lipids were found to be sensitive by the ATP injection and this represented by the detection of symPO2vibrational mode in all ATP injected groups relative to control group. We concluded that ATP injection dramatically affect both protein and lipid moieties of lens fiber. Recommendation of a regular examination for whole eye especially lens in case of retinal degeneration not retina only.