This study aims to compare physico-chemical parameters of the water quality between hot springs and borehole waters. Twenty samples were collected from two regions Singida and Dodoma of Tanzania. Multimeter used for the analysis of physical parameters pH, EC, TDS, salinity and turbidimeter used to analyseturbidity. Titrimetric methods were used for chemical parametersCl^-, total hardness, Ca2+ and Mg2+. UV-Visible spectrophotometric methods used for the analysis of NO_3^-,〖 SO〗_4^(2-),F^-, Fe2+and Mn2+ and Flame Atomic Absorption methods for Cd2+, Zn2+, Ni2+, Cu2+ and K+. The parameters EC, TDS, salinity, turbidity,Cl^-, NO_3^- , 〖 SO〗_4^(2-), F^-and Mn2+are higher (EC = 508.33-4790 µS/cm, TDS = 249.33-2349.30 mg/L, salinity = 0.27-2.53 ppt, turbidity = 0.81-513.73 NTU,Cl^-= 54.32-900.52 mg/L, NO_3^-= 0.1-63.30 mg/L, 〖 SO〗_4^(2-)=38.33-343.33 mg/L, F^-=0.47-9.5 mg/L and Mn2+= 0.6-1.9 mg/L) in hot springs than borehole waters (EC = 844.0-1821.67 µS/cm, TDS = 414.67-891.33 mg/L, salinity = 0.4-0.87 ppt, turbidity = 3.8-147.9 NTU,Cl^- = 119.68-356.50 mg/L, NO_3^- = 0.93-16.78 mg/L, 〖 SO〗_4^(2-) = 42.0-128 mg/L, F^-= 0.18-1.38 mg/L and Mn2+ = 1.5-1.6 mg/L). But other parameters Total hardness, Ca2+, Mg2+, Fe2+ and Ni2+ are higher in borehole waters than hot springs.The t-test (p=0.05) showed that there is significant difference of the parameters〖 SO〗_4^(2-),F^- and Ni2+ between hot spring and borehole waters. Based on this study it is observed that some of the parameters are at higher levels than permissible values for both hot spring and borehole waters. Therefore, there is a need of treatment for these waters before using for domestic purposes.