Effect of cpp- acp, polarized enamel using hydrogen peroxide on remineralizing potential of bovine - an ex-vivo study

Demineralization of enamel surrounding orthodontic brackets is a serious clinical problem and these conditions are treated by the use of remineralization creme. The effectiveness of the regimens can be determined by measuring the Bone Mineral Density (BMD) and Bone Mineral Content (BMC). In general these parameters are studied using polarized light, desktop micro CT. In the present ex vivo study, effectiveness of remineralization creme namely Casein Phosphopeptide Amorphous Calcium Phosphate (CPP-ACP), and enamel surface of bovine anterior teeth treated with hydrogen peroxide was analyzed using dual X-ray absorbtiometry (DXA). 52 samples of bovine anterior teeth were collected from slaughter house and cleaned as per the standard procedures. The collected samples were subjected to demineralization using a demineralizing buffer solution. Subsequently, the teeth samples were remineralized with CPP-ACP or hydrogen peroxide treatment and curing with light cure unit, stored in artificial saliva. The BMD and BMC in control and experimental group samples were measured using non-invasive DXA at regular intervals. The BMC of the sample treated with hydrogen peroxide was more when compared to the sample treated with CPP-ACP. However, no significant changes in BMD contents were observed among the experimental groups. No significant changes were seen in terms of their lean body mass and total fat content among the teeth of control and experimental groups. The remineralization potential is more when teeth were treated with hydrogen peroxide and light cure when compared to CPP-ACP.