Genotyping of p53 exon 4 codon 72 in sudanese patients with lymphoid leukaemias

The aim of this was to investigate the association between P53 exon 4 codon 72 genotypes and acute and chronic lymphoid leukaemias. A total of 77 subjects were enrolled in this study, 32 with acute lymphoblastic leukaemia, 15 with chronic lymphocytic leukemia and 30 healthy individuals as a control group. Genomic DNA was extracted from patients’ blood samples by salting out method, and analyzed for determination of P53 exon 4 codon 72 genotypes using-allele specific polymerase chain reaction (AS-PCR). The results showed that, the genotype Arg/Pro was the most frequent (75%) in patients with ALL, followed by the genotype Arg/Arg (19%), and Pro/Pro (6%). Also in patients with CLL the genotype Arg/Pro was the most prevalent (67%), followed by Arg/Arg (27%), and Pro/Pro (6%). In healthy control the Arg/Arg genotype was the most frequent genotype (80%), followed by Arg/Pro (17%), and Pro/Pro (3%). There was a significant association between the Arg/Pro genotype and both ALL (OR: 4.5, CI: 1.97-10.27, P .value: 0.000) and CLL (OR: 4.0, CI: 1.7-9.6, P.value:0.001). In conclusion, Arg/Pro genotype might confer increased risk for development of ALL and CLL.