Influence of spray application of n-(phosphonomethyl) glicine on morphoanatomical characters of psoralea corylifolia l.

The plant of Psoralea corylifolia sprayed with glyphosate (N-(phosphonomethyl)glycine’) showed some morphological changes such as scorching, wilting of leaves and stems, crumpling and yellowing of leaves and drying of all the parts of the plant at all concentrations (100,200,400,600 ,800, 1000 and 1200ppm). On fourth day of spraying plant showed yellow spots on lower leaves at all concentrations. When glyphosate sprayed at concentration of >= 400ppm lamina showed burning effects and they dried on completely. Stem became pale green on eight day after spraying. At 600ppm and above concentration, apical and lateral growth was found inhibited, whereas at 1200ppm the leaf dried completely, thus this dose acts as a lethal dose to this plant. Glyphosate induced anatomical changes in stem, root, petiole and leaf. At 400ppm phloem showed abnormal meristematic activity owing to periclinal and anticlinal divisions of phloem, the cortex was pushed outward and crushed. The xylem and pith cells disintegrated at many places and formed lacunae at 800ppm. Similarly at 800ppm root showed ruptured epidermis and disorganization of cortex forming lacunae. At 1000ppm, the leaf epidermis showed cellular disorganization of the spongy and palisade tissue.