Invitro cytotoxic evaluation of annona muricata (graviola) leaves on mcf-7 cell line

Annona muricata is a tropical plant which has been utilized as a folk medicine to treat many diseases including cancer and many other bioactivities. The aim of this study was to evaluate the phytochemicals present in the leaf extracts and to analyze its medicinal importance in cancer therapy. Additionally, the study determined the mineral elements such as Fe and Mg which are the structural component of tissues and essential for the functioning of all the cells. Vitamin C is a well-known antioxidant that helps to strengthen immune system by reducing the amount of free radicals in the body. A. muricata leaf extracts were evaluated for the scavenging assay (DPPH;1-1-diphenyl-2- picryhydrazyl), (FRAP-Ferric Reducing Antioxidant Power) and the study shows that the leaf extracts have good scavenging activity. The antibacterial activity of leaf extract was examined by disc diffusion method in Gram negative and Gram-positive bacterium. Invitro test against breast cancer cell growth was conducted using the MCF-7 cell line by means of the MTT-assay. Ethanol leaf extract have highest anticancer activity with an IC50 value of 141.56µg/ml. The results obtained from the analysis showed that the leaves of A. muricata has the property to inhibit the cell growth and it has medicinal properties and could be good source of drugs for treating cancer.