Optimization of Process Parameters for the Production of Thermostable Laccase by Pleurotus flabellatus ATK-1 using Response Surface Methodology

Optimization of laccase production was carried out using a new strain, Pleurotus flabellatus ATK-1 with three independent process parameters viz glucose, incubation time and pH using full factorial central composite experimental design. The optimum values for the process parameters for the maximum laccase production were obtained glucose 2.8%, incubation time 12.6 days and pH 6.7. In the work, we have demonstrated the use of a central composite design for maximum enzyme production by optimizing the process parameters.