Study of some immunoenzymatic parameters in pediatric ischemic stroke

Background: Ischemic stroke (IS) in children is a major neurological emergency, being a primary cause of morbidity and mortality. It is detected in the neonatal period in one of 2500 – 4000 live births, and in children over one month of age it is found at 1,2 – 8 per 100000. Aim: Studying of immunoenzymatic parameters in IS in children for the assessment of pathogenesis, early diagnosis and predictive factors of the disease. Materials and methods: During 2017 – 2019 in the Republic of Moldova, was carried out a prospective study on a sample of 53 children with IS, which are formed study sample (SS), in which in the acute phase of the process using ELISA immunoassay were analyzed the serum levels of the following markers: endoglin CD105 (ENG), antiphospholipid antibodies (APA) and interleukin-6 (IL-6). These markers were also assessed in 53 practically healthy children which formed control sample (CS). Results: The average values of immune markers in the acute period of IS were as follows: (1) ENG – 2,06±0,012 ng/ml (F=84,812, p<0,001); (2) APA – 11,37±0,046 U/ml (F=60,701, p<0,001); (3) IL-6 – 22,02±2,143 pg/ml (F=43,810, p<0,001), which were significantly different from the values detected in practically healthy children. Conclusions: In children with IS in the acute period some immunological parameters changes which show the role of these parameters as biomarkers which are involved in ischemic processes of the brain. New longitudinal studies in children with IS may improve early diagnosis and treatment of these children.