In vitro adventitious root and hairy root cultures in boerhaavia diffusa l.

Boerhaavia diffusa L. is a valuable medicinal plant indigenous to India commonly called Punarnava. Adventitious roots were initiated from the leaf explants on a media containing Murashige and Skoog media supplemented with various concentrations (0.25, 0.5, 1.0, 2.0 & 4.0 mg/L) of NAA and IBA. Maximum number of roots were obtained on MS media supplemented with 1.0 mg/L NAA, while profuse rooting was also found in media supplemented with 2.0 mg/L and 4.0 mg/L NAA. In vitro grown roots were transferred to liquid culture media containing NAA for root proliferation. The cultured roots were harvested after 40 days. Agrobacterium rhizogenes mediated transformation was carried out with in vivo leaf explants of B. diffusa. Hairy roots were obtained on a medium containing hormone free MS medium.