Extraction of phenolic compounds from pinus patula bark using ethanol-water mixtures and the anti-inflammatory action of the ethanolic extract

Interaction between factors to take into consideration for extraction of bioactive phenolics compounds from Pinuspatula bark was evaluated. Contact time, particle size, temperature, ethanol: water ratio, bark: solvent ratio and revolutions per minutewere evaluated to identify the highest percentage of extraction. The optimal extraction conditions resulting in the highest percentage of phenolics and procyanidins compounds were 6 hours of contact, less than 1.18 mm of particle size, with a temperature of 60°C, and ethanol: water ratio of 30:70, and bark: solvent ratio of 1:10 g:mL, with 250 revolutions per minute. The combination of this factors resulted in 8.56 ± 0.86% of extract of Pinuspatula bark, with 1610 μg/g of total phenols and 560.82 μg/g of procyanidins. Concerning their anti-inflammatory activity, P. patula bark extract exhibited a broad activity towards eicosanoids concentration produced by the polymorph nuclear leukocytes of rats. Results indicated that the hydroalcoholic extract of Pinuspatula bark has anti-inflammatory activity. ANOVA analysis showed significant differences between treatment groups (p < 0.05) receiving drug (Ketoprofen®) versus ethanolic extract (Pinuspatula bark).