Analytical method development and validation of sitagliptin in bulk and tablet formulation by uv spectrophotometry

Sitagliptin phosphate is antidiabetic drug. A simple UV Spectrophotometric method was developed for the determination of Sitagliptin using distilled water and linearity was observed in the concentration range of 10 to 100µg /ml. The proposed method was statistically validated. From the results obtained for precision, it was found that % RSD is less than 2% indicating that the proposed method has good reproducibility. For Accuracy, it was found that Percentage Recovery values of pure drug from the analyzed formulation were in between 99 - 101% which indicates that the method is accurate and commonly used excipients and additives present in the formulation were not interfering in the proposed method. The quantification was achieved by the spectroscopy method at 267 nm for Sitagliptin phosphate.