Ameliorative effects of Moringa oleifera Lam. leaf extract against systemic immune effects of titanium dioxide nanoparticles in male albino rats

Introduction and aim of the work: The protective effects of Moringa oleifera leaves extract (MOLE) against orally administered titanium dioxide nanoparticles (TiO2 NPs) for 3 months on male albino rats were examined. Methods: The rats were divided into five groups • Group 1: rats were orally administrated with saline as a negative control group, • Group 2: Rats were orally administrated with TiO2 (1200 mg kg−1) (1\10 LD50), Group 3: Rats were administrated orally by MOLE (50 mg kg−1 BW), Group 4: co-treatment of MOLE and TiO2 as a protective group for 3 months and Group 5: post treatment of TiO2 by MOLE for 3 successive month as therapeutic treatment. Spleen was examined for alterations in cytokines expression as CD3 and TNF-α, histopathology and histochemically by estimation of total proteins and polysaccharides. Results: TiO2 NPs up-regulated CD3 and TNF-α expression in spleen where TNF-α expression was significantly increased (p<0.05) and CD3 expression was significantly (P<0.05) decreased but MOLE administration as co-treatment or therapeutic normalized cytokines expression. Conclusion: present findings confirmed the protective and therapeutic effects of MOLE on TiO2 NPs induced alteration in immune responses in male albino rats.