Potential alternative to formaldehyde fixatives - A study

Aim: The aim of this study is to find a potential alternative that can be used in place of the regular fixatives. Materials and Method: In this study, we will use sodium nitrite as a fixative. 5 samples of the tissue overlying the extracted teeth will be used. 30g, 35g, 40g, 45g and 50g of sodium nitrite were the concentrations. Background: Fixation is the foundation step behind the study of pathology and essentially exists to prevent the autolysis and degradation of the tissue and tissue components such that they can be observed both anatomically and microscopically following sectioning. The broad objective of tissue fixation is to preserve cells and tissue components in a “life-like state”. Sodium nitrite has been found to inhibit growth of disease-causing microorganisms; give taste and color to the meat; and inhibit lipid oxidation that leads to rancidity. The study was carried out in 2016 using 40g, 50g, 60g and 80g of sodium nitrite in 100 ml of water. 40g sodium nitrite showed the best fixation properties among the other concentrations. Even 60g showed significant results. However in the experimental study we chose concenterations around 40g. Conclusion: An innovative Method to detect the effectiveness of a new salt like sodium nitrite as a fixative in place of the usual toxic Formalin.